Its solubility is 0.77 grams per liter (g/l) in water and 2.9 g/l in ethanol. Hence, the relative concentration of one form, which varies as a function of … Phenol red was used by Leonard Rowntree and John Geraghty in the phenolsulfonphthalein test to estimate the overall blood flow through the kidney in 1911. Phenol red has been used as a pH indicator dye in tissue culture media for decades (Figure 1). A solution of phenol red is used as a pH indicator, often in cell culture. Search for: List of Vaccines. [6], In several sources, the structure of phenol red is shown with the sulfur atom being part of a cyclic group, similar to the structure of phenolphthalein. Standardized for colorimetric pH determination. Its color exhibits a gradual transition from yellow to red over the pH range 6.8 to 8.2. I. Its solubility is 0.77 grams per liter (g/L) in water and 2.9 g/L in ethanol. Additionally, phenol red tubes contain an inverted Durham tube. A solution of phenol red is used as a pH indicator: its color The pH of blood ranges from 7.35 to 7.45, for instance. Its color exhibits a gradual transition from yellow (λmax = 443 nm[2]) to red (λmax = 570 nm[3]) over the pH range 6.8 to 8.2. When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. Many biologists find this a convenient way to rapidly check on the health of tissue cultures. Bottle/50 mL spec. Congo Red (powder) C19H13NaO5S. Add all three to Cart Add all three to List. �8�z0E00�1�2:+0(�{0�0IU1�jPO`d�R`�a���ʰ����QoC��5�8y��[)p]`�����ɰ��"%�Кp;��?�i��H�c�; e�bVf[(�@� Р!# They are used to visually signal the acidity or alkalinity of an aqueous (water-based) solution. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. Negative:The tube containing medium will remain red, indicating the bacteria cannot ferment that particular carbohydrate source present in the media.II. pH indicators generally change color over a range of two pH units. 13 0 obj <> endobj HY-D0169A. Above pH 8.1, phenol red turns a bright pink (fuchsia) color. Phenol red is a weak estrogen mimic, and in cell cultures can enhance the growth of cells that express the estrogen receptor. A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. This form is sometimes symbolically written as H+2PS− and is orange-red. Albert P Li. Phenolsulfonphthalein or otherwise called phenol red is a pH indicator commonly used in cell biology laboratories. At still higher pH (pKa = 7.7), the phenol's hydroxy group loses its proton, resulting in the red ion denoted as PS2−. Depending on the intended cell type different mixtures of salts, amino acids, vitamins, buffers, carbohydrates, fatty acids, and supplements such as serum are used. Frequently bought together + + Total price: $29.48. Standardized for colorimetric pH determination. endstream endobj startxref Cite. Phenol red exists as a red crystal that is stable in air. Phenol Red Indicator Solution: Dissolve 0.1 g of phenol red in 2.82 ml of 0.1 M sodium hydroxide and 20 ml of ethanol (95 percent). Previous. 59 0 obj <>/Filter/FlateDecode/ID[<460C89CDBDC0009432C14715F39BAA36><2BA3B43A4554254491AFA26C4B8FE729>]/Index[13 114]/Info 12 0 R/Length 172/Prev 212768/Root 14 0 R/Size 127/Type/XRef/W[1 3 1]>>stream High levels of bromine can convert phenol red to bromophenol red (dibromophenolsulfonephthalein, whose lowered pKa results in an indicator with a range shifted in the acidic direction – water at pH 6.8 will appear to test at 7.5). DTXSID2044420. Phenol red is yellow at a pH < 6.8 and red at a pH of > 7.4, therefore if a bacterium ferments a sugar to acid a yellow color will develop. That I am using is " Phenol Red indicator, ACS reagent, CAS Number 143-74-8 ". While its presence is not critical for maintaining cell cultures, it is often used as a quick means for researchers to check on culture stock… Phenol Red pH indicator Synonym: Phenolsulfonphthalein CAS Number 143-74-8. Typically, 15 mg/l are used for cell culture. Even higher levels of bromine (>20 ppm) can result in the secondary conversion of bromophenol red to bromophenol blue with an even lower pKa, erroneously giving the impression that the water has an extremely high pH despite being dangerously low. Find chemicals for your experiments at Home Science Tools! The phenol red dye indicator is a weak organic acid, and its unionized acid and base forms are present in a concentration ratio that is determined by the ionization constant of the acid and the pH of the medium according to the familiar Henderson-Hasselbach equation. '�.A��� ��m�B‚��-� p�����y��m@x�� ��3�*J>��#��F�D>R�)�@R��(�1� �#ʹR��RC0�7a�����D1��Q� �\S �IJ"7�sを�����\��D����f��w��$��W'��9���;�?�Ҿ�儝�)Z9���CD��� u�z�aXHH^� � Ԏ�e��6v��w8�.�R�h�I��%5� �Xh��G ����X�fcу�X 7�{=�ޛ���A�B ����+��oڑ�B@����HO���.���~�ٴ#��~��.�I�%¢����'N�䃂H;”�v�wi�'�{IZ��B@�i=�큥٠�(y����G;�|�!=a@H�f�. Phenol Red Indicator Solution. In addition, the waste products produced by the mammalian cells themselves will slowly decrease the pH, gradually turning the solution orange and then yellow. Phenol Red sodium salt, ACS reagent. Principle: Phenol Red Fermentation medium contains peptone, phenol red (a pH indicator) and the carbohydrate to be tested. Formula: C₁₉H₁₄O₅S MW: 354.38 g/mol Storage Temperature: Ambient: MDL Number: MFCD00003552 CAS Number: 143-74-8: Order Now. The pH of blood ranges from 7.35 to 7.45, for instance. Many of these defined mixtures also contain phenol red. A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. In the event of problems, waste products produced by dying cells or overgrowth of contaminants will cause a change in pH, leading to a change in indicator color. Acid production: 1. This color change is an indication that even in the absence of contamination, the medium needs to be replaced (generally, this should be done before the medium has turned completely orange). The transition range may shift slightly depending on the concentration of the indicator in the solution and on the temperature at which it is used. [11] It has been used to induce ovarian epithelial cells from post-menopausal women to differentiate into cells with properties of oocytes (eggs), with potential implications for both fertility treatment and stem cell research. Phenol red salt is used routinely as pH indicator. If the pH is increased (pKa = 1.2), the proton from the ketone group is lost, resulting in the yellow, negatively charged ion denoted as HPS−. It is a weak acid with pKa= 8.00 at 20°C. Molecular Weight 354.38 . February 28, 2019 admin Ingredients 0. [12], Phenol red, sometimes labelled with a different name, such as "Guardex Solution #2", is used as a pH indicator in home swimming pool test kits. Above pH 8.2, phenol red turns a bright pink (fuchsia) color. Phenol red is a pH indicator. Phenol Red, sodium salt, pure, water soluble, indicator. Calculations like this one might make a good project for a college freshman chemistry class. �g���_p�����zq���D_�2������{�������볷W�W�}~������W�~����˿�}~���_~�8{�⮗����خ��O�8�� @N���/O����΀���=����?�������Y �:��l��ǧo�/~����o�g�. [4][5], In crystalline form, and in solution under very acidic conditions (low pH), the compound exists as a zwitterion as in the structure shown above, with the sulfate group negatively charged, and the ketone group carrying an additional proton. PHENOL RED INDICATOR AR (pH INDICATOR) (PH 6.4-8.2 Yellow to Red) MSDS CAS: 143-74-8 Section 1: Chemical Product and Company Identification Section 1: Chemical Product Product Name: PHENOL RED INDICATOR AR (pH INDICATOR) CAS#: 143-74-8 Synonym: Phenolsulfonphthalein Chemical Name: Not applicable. It is yellow below 6.8 pH and bright fushia (pink) above 8.2 pH. Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. Phenol Red is used as a pH indicator in cell culture applications. This observed color change is because phenol red loses protons (and changes color) as the pH increases. Beilstein/REAXYS Number 326470 . Phenol Red Indicator Structure Search; Print SDS. Polio (IPV – Ipol) Next. A solution of phenol red is used as a pH indicator: its color exhibits a gradual transition from yellow to red over the pH range 6.6 to 8.0. This usually results in an acidification of the medium, turning it yellow. Phenol red SDS Safety Data Sheet for Phenol red 107241. It indicates that there is drop in the pH because of the production of the acid by the fermentation of the carbohydrate (sugar) present in the media. It is a weak acid with pK a = 8.00 at 20 °C (68 °F). DSSTox_GSID_44420. Most living tissues prosper at a near-neutral pH; that is, a pH close to 7. %%EOF Specification Test Results. Phenol Red Indicator Solution. Phenolsulfonphthalein is being investigated for use clinically due to its weak estrogen mimicking actions. 2. Phenol Red, sodium salt. Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. In Vitro ADMET Laboratories, LLC. grade. Glycerin. For example, a culture of relatively slowly dividing mammalian cells can be quickly overgrown by bacterial contamination. Bottle/50 mL spec. Phenol red exists as a red crystal that is stable in air. 0 Bovine Brain Endothelial Cell Growth Medium without Phenol Red (500 ml) 1 Product Result Indicators usually exhibit intermediate colors at pH values inside the listed transition range. 3 The two forms of the dye have different optical absorption spectra. The phenol red indicator is discussed. There are numerous formulations for in vitro cell propagation. By measuring the amount of phenol red excreted colorimetrically, kidney function can be determined.[10]. Phenol Red sodium salt, powder. Phenol red exists as a red crystal that is stable in air. [13], Chlorine can result in the bleaching of the dye in the absence of thiosulfate to inhibit the oxidizing chlorine. Color range: pH 6.0-8.5. Chemical Formula: C 19 H 14 O 5 S Brand: OXFORD Phenol red is an indicator commonly used to measure pH in swimming pool test kits, see e.g. (A table of other common chemical indicators is available in the article on pH indicators.). [8], Several indicators share a similar structure to phenol red, including bromothymol blue, thymol blue, bromocresol purple, thymolphthalein, and phenolphthalein. KS-00000XAO. Empirical Formula (Hill Notation) C 19 H 14 O 5 S . MW 354.37. pH indicator widely used in culture media to identify pH changes from neutral (red) to acidic (yellow) values. Phenol red is an acid-base indicator. Phenol Red Indicator, ACS reagent, CAS Number: 143-74-8,(32661 Fluka).Shop now or request a quote. CHEMBL3187327. phenol red indicator. Phenol red is frequently used in cell culture media as a pH indicator. [14], InChI=1S/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19/h1-12,20-21H, O=S2(=O)OC(c1ccccc12)(c3ccc(O)cc3)c4ccc(O)cc4, Except where otherwise noted, data are given for materials in their, "Differences between phthaleins and sulfonphthaleins", "Molecular Structure of the Zwitterionic Form of Phenolsulfonphthalein", "THE PHENOLSULPHONEPHTHALEIN TEST FOR ESTIMATING RENAL FUNCTION", "Phenol red in tissue culture media is a weak estrogen: Implications concerning the study of estrogen-responsive cells in culture", "Oogenesis in Cultures Derived from Adult Human Ovaries", Guardex Solution 2 - Phenol Red Material Safety Data Sheet, Effect of Bromine on Phenol Red in pH Tests, Video of phenol red activity demonstration and medium preparation, 4'-O-β-D-Glucosyl-9-O-(6''-deoxysaccharosyl)olivil, https://en.wikipedia.org/w/index.php?title=Phenol_red&oldid=999029658, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, This page was last edited on 8 January 2021, at 04:19. 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